The legal requirement to wear face coverings in indoor public spaces and on public transport becomes guidance today (Monday).
However, First Minister Nicola Sturgeon has said she is confident Scots will continue to wear face masks after rules are eased.
The move had been delayed twice amid record levels of Covid cases and high numbers of people in hospital with the virus.
With the infection rate now falling across the country, the government has confirmed the laws will be lifted.
Speaking to the Press Association on Sunday, Sturgeon said: “I’m not saying every single person will (continue to wear masks), people will make their own decisions.
“But I think, just as the vast majority of people have abided by all that we’ve asked of them over the last two years – not because politicians have been asking or the law necessarily has required it – because people understand that the best way to protect themselves and protect those they love is to abide by these really sensible, basic precautions against the spread of a virus.
“We can’t keep things in law beyond a point where that is deemed to be proportionate, so we will move the face covering requirement out of law and into guidance.”
The First Minister said Covid-19 was “still out there”, adding: “Wearing a face covering is a bit of protection you can give, not only to yourself but to the people you might be around – including people who might be more clinically vulnerable.”
She continued: “Yes, I’ve got confidence in the good sense and the responsibility of the people across Scotland.”